Journal: Oncotarget

Article Title: Imidazoline I 2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway

doi: 10.18632/oncotarget.15472

Figure Lengend Snippet: ( A ) Liver fibrosis as assessed by hematoxylin and erosin (H&E), Sirius red Staining and Masson trichrome staining ( n = 6 in each group). Fibrosis is represented by collagen deposition (red color area) in Sirius Staining and (blue color area) in Masson's trichrome staining. ( B ) Trends in the body weights of C57BL/6 mice were monitored at 1 week intervals throughout the 8 weeks of CCl 4 treatment. ( C ) The liver weights and liver/body weight ratio at 8 weeks were measured. ( D ) The protein levels of IL-1β, IL-6, TNF-α and TGF-β in liver tissue homogenates were measured by ELISA. The experiments were repeated for three times and data are represented as mean ± SEM. # p < 0.05 versus Control; ## p < 0.01 versus Control; * p < 0.05 versus CCl 4 ; ** p < 0.01 versus CCl 4 .

Article Snippet: The concentrations of IL-1β, IL-6 and TNF-α in liver tissue homogenates were measured by mouse ELISA kits (Wuhan Boster Biological Technology Co., Ltd.).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Oncotarget

Article Title: Imidazoline I 2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway

doi: 10.18632/oncotarget.15472

Figure Lengend Snippet: LX2 cells were transfected with control shRNA or Nrf2 shRNA Lentivirus Particles, then treated with IDA (100 μM) for 1 h and next treated with or without TGF-β (5 ng/ml) for 12 h. ( A ) The expressions of HO-1 and NQO-1 were detected by western blotting. ( B ) The relative enzyme activity of SOD and GPx were measured by ELISA. ( C ) The expressions of p-Smad2, Smad2, p-Smad3, Smad3, α-SMA and Col1 were detected by western blotting. ( D ) The expression of α-SMA was measured by immunofluorescence assay. Data are represented as mean ± SEM. * p < 0.05 vs TGF-β; n.s. = No significant. Statistical analyses were performed using Student's t test.

Article Snippet: The concentrations of IL-1β, IL-6 and TNF-α in liver tissue homogenates were measured by mouse ELISA kits (Wuhan Boster Biological Technology Co., Ltd.).

Techniques: Transfection, Control, shRNA, Western Blot, Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence